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guinea pig anti-map2  (Synaptic Systems)


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    Structured Review

    Synaptic Systems guinea pig anti-map2
    Guinea Pig Anti Map2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-map2+guinea+pig/anti+map2/pm40531619-160-2-9
    Average 90 stars, based on 1 article reviews
    guinea pig anti-map2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Staining:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Fluorescence:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Microscopy:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Expressing:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Over Expression:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Binding Assay:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Plasmid Preparation:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Confocal Microscopy:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Inhibition:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).

    Activity Assay:

    Article Title: Electrochemical Roughening and Carbon Nanotube Coating of Tetrodes for Chronic Single-Unit Recording
    Article Snippet: Hippocampal neurons were fixed with 4% PFA for 8 mins and then gently incubated with PBS 1X for 2 mins and then with a solution containing 10% horse serum, 0.1 mM glycine, and 0.1% Triton X-100 in Hanks’ balanced salt solution two times for 5 min. Pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems GmbH, Göttingen, Germany; 1:1,000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: JNK and PI3K signaling pathways mediate synapse formation and network spontaneous activities in primary neurons
    Article Snippet: The subsequent antibodies were employed at the specified concentrations: anti-MAP2 Guinea pig (Synaptic Systems; Cat# 188004; 1:1000); anti-VGAT mouse (Synaptic Systems; Cat# 131011; 1:1000); anti-VGluT1 rabbit (Synaptic Systems; Cat# 135316; 1:1000); anti-PSD95 mouse (Thermo Fisher; Cat# MA1046; 1:500); phospho-CREB (Cell Signaling Technology; Cat# 9198S; 1:1000); phospho-JNK (Cell Signaling Technology; Cat# 9255S; 1:2000); phospho-Akt (Cell Signaling Technology; Cat# 1911; 1:2000); Goat anti-Guinea Pig Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher; Cat# A11037; 1:1000); Goat anti-Rabbit Secondary Antibody, Alexa FluorTM Plus 647 (Thermo Fisher; Cat# A32733; 1:1000); Goat anti-Mouse Secondary Antibody, Alexa FluorTM 546 (Thermo Fisher; Cat# A11030; 1:1000).

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: Other primary antibodies used were: anti-TRAF6 rabbit (Santa Cruz, #sc-7221; 1:100), anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4◦C.

    Article Title: The Interaction of TRAF6 With Neuroplastin Promotes Spinogenesis During Early Neuronal Development.
    Article Snippet: To visualize dendritic protrusions, after transfection, pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich, #11814460001; 1:1,000) antibodies.

    Article Title: TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin
    Article Snippet: Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems, #162 204; 1:1,000), anti-Homer1 mouse (Synaptic Systems, #160 011; 1:500), anti-RelA (p65) rabbit (Santa Cruz, #sc-372; 1:500); anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) primary antibodies for overnight at 4°C.

    Article Title: The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour
    Article Snippet: Secondary antibodies: (1:3000) horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse (Vector Labs, #PI1000, Cat no. PI2000). . Immunohistochemistry Primary antibodies: anti-MAP2 mouse (1:500, Sigma M9942), anti-MAP2 guinea pig (1:500, Synaptic System, 188 004), anti-GFP chicken (1:5000, Abcam ab13970), anti-BrdU rat (1:250, Biorad, OBT0030), anti-Cre rabbit (1:1000, Abcam ab41104), anti-FOXP2 (1:1000, Abcam ab16046), anti-NeuN mouse (1:1000, Millipore MAB377), anti-Cux1 rabbit (1:1000, Santa Cruz, sc13024), anti-CTIP2 rat (1:1000, Abcam ab18465) and anti-pS6 rabbit (1:500, Cell Signaling #2211).



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    guinea pig anti-map2 monoclonal antibody - by Bioz Stars, 2026-09
    90/100 stars
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    Synaptic Systems guinea pig anti-microtubule-associated protein 2 (map2)
    Absent in melanoma 2 (AIM2) promotes the development of bone cancer pain (BCP). ( A ) Double immunofluorescence staining with anti-AIM2 (red) and <t>anti-microtubule-associated</t> <t>protein</t> <t>2</t> <t>(MAP2)</t> (cyan) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) The measurement of paw withdrawal threshold (PWT) was assessed on days 0, 3, 7, 10, 14, 17, and 21 following the surgery in sham, BCP, BCP + CON- Lentivirus (LV), and BCP + AIM2-LV mice. Following BCP, the PWT in mice decreased, and the hypersensitivity to BCP was reduced by inhibiting AIM2 ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + CON-LV; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Immunofluorescence staining with anti-AIM2 (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups mice. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( D ) Quantitative analysis of AIM2 expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV and BCP + AIM2-LV groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. All the blots are original Western blots. n represents the number of experimental animals in each group.
    Guinea Pig Anti Microtubule Associated Protein 2 (Map2), supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-map2+guinea+pig/anti+map2/pmc11669570-60-23-28
    Average 90 stars, based on 1 article reviews
    guinea pig anti-microtubule-associated protein 2 (map2) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Synaptic Systems guinea-pig anti-map2
    Absent in melanoma 2 (AIM2) promotes the development of bone cancer pain (BCP). ( A ) Double immunofluorescence staining with anti-AIM2 (red) and <t>anti-microtubule-associated</t> <t>protein</t> <t>2</t> <t>(MAP2)</t> (cyan) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) The measurement of paw withdrawal threshold (PWT) was assessed on days 0, 3, 7, 10, 14, 17, and 21 following the surgery in sham, BCP, BCP + CON- Lentivirus (LV), and BCP + AIM2-LV mice. Following BCP, the PWT in mice decreased, and the hypersensitivity to BCP was reduced by inhibiting AIM2 ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + CON-LV; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Immunofluorescence staining with anti-AIM2 (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups mice. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( D ) Quantitative analysis of AIM2 expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV and BCP + AIM2-LV groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. All the blots are original Western blots. n represents the number of experimental animals in each group.
    Guinea Pig Anti Map2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-map2+guinea+pig/anti+map2/pm39209987-187-28-30
    Average 90 stars, based on 1 article reviews
    guinea-pig anti-map2 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Absent in melanoma 2 (AIM2) promotes the development of bone cancer pain (BCP). ( A ) Double immunofluorescence staining with anti-AIM2 (red) and anti-microtubule-associated protein 2 (MAP2) (cyan) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) The measurement of paw withdrawal threshold (PWT) was assessed on days 0, 3, 7, 10, 14, 17, and 21 following the surgery in sham, BCP, BCP + CON- Lentivirus (LV), and BCP + AIM2-LV mice. Following BCP, the PWT in mice decreased, and the hypersensitivity to BCP was reduced by inhibiting AIM2 ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + CON-LV; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Immunofluorescence staining with anti-AIM2 (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups mice. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( D ) Quantitative analysis of AIM2 expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV and BCP + AIM2-LV groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. All the blots are original Western blots. n represents the number of experimental animals in each group.

    Journal: Scientific Reports

    Article Title: AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice

    doi: 10.1038/s41598-024-83027-w

    Figure Lengend Snippet: Absent in melanoma 2 (AIM2) promotes the development of bone cancer pain (BCP). ( A ) Double immunofluorescence staining with anti-AIM2 (red) and anti-microtubule-associated protein 2 (MAP2) (cyan) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) The measurement of paw withdrawal threshold (PWT) was assessed on days 0, 3, 7, 10, 14, 17, and 21 following the surgery in sham, BCP, BCP + CON- Lentivirus (LV), and BCP + AIM2-LV mice. Following BCP, the PWT in mice decreased, and the hypersensitivity to BCP was reduced by inhibiting AIM2 ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + CON-LV; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Immunofluorescence staining with anti-AIM2 (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups mice. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( D ) Quantitative analysis of AIM2 expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV and BCP + AIM2-LV groups on day 7 post-operation. β-Actin and GAPDH were used as loading controls, respectively. All the blots are original Western blots. n represents the number of experimental animals in each group.

    Article Snippet: The antibodies used were as follow: mouse anti-AIM2 monoclonal antibody (1:300; cat. no.: 66902-1-IG, RRID: AB_2882230; Proteintech, USA) ; rabbit anti-AIM2 polyclonal antibody (1:300; cat. no.: bs-5986R, RRID: AB_11074508; Bioss, China) ; mouse anti-GluN1 monoclonal antibody (1:300; cat. no.: 32-0500, RRID: AB_86917; Thermo Fisher Scientific, USA) ; rabbit anti-STING polyclonal antibody (1:300; cat. no.: 19851-1-AP, RRID: AB_10665370; Proteintech, USA) ; mouse anti-Synapsin1 monoclonal antibody (1:300; cat. no.: MA5-31919, RRID: AB_2787542; Thermo Fisher Scientific, USA); guinea pig anti-MAP2 monoclonal antibody (1:300; cat. no.: 188004, RRID: AB_2138181; Synaptic Systems, Germany); mouse anti-Iba1 antibody (1:200; cat. no.: sc-32725, RRID: AB_667733; Santa Cruz, USA).

    Techniques: Double Immunofluorescence Staining, Staining, Immunofluorescence, Injection, Virus, Fluorescence, Transfection, Expressing, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR

    Stimulator of interferon gene (STING) agonist 5,6-dimethyl-9-oxo-9H-xanthene-4-acetic acid (DMXAA) alleviates hyperalgesia of mice with bone cancer pain (BCP). ( A ) Double immunofluorescent staining with anti-microtubule-associated protein 2 (MAP2) (cyan) and anti-STING (red) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) BCP mice were treated with intraperitoneal (i.p.) injection of DMXAA twice on days 3 and 6. The Paw withdrawal threshold (PWT) of mice was detected on 0, 3 (before DMXAA injection), 6 (before DMXAA injection), 10, and 14 days after the surgury in sham, BCP, BCP + vehicle, and BCP + DMXAA groups. DMXAA treatment attenuated hypersensitivity induced by BCP ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + vehicle; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Quantitative analysis of STING expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + vehicle; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of the sham, BCP, BCP + vehicle, and BCP + DMXAA groups on day 7 post-operation. α-Tubulin and GAPDH were used as loading controls, respectively. n represents the number of experimental animals in each group.

    Journal: Scientific Reports

    Article Title: AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice

    doi: 10.1038/s41598-024-83027-w

    Figure Lengend Snippet: Stimulator of interferon gene (STING) agonist 5,6-dimethyl-9-oxo-9H-xanthene-4-acetic acid (DMXAA) alleviates hyperalgesia of mice with bone cancer pain (BCP). ( A ) Double immunofluorescent staining with anti-microtubule-associated protein 2 (MAP2) (cyan) and anti-STING (red) in the right spinal dorsal horn of the wild type (WT) mice. DAPI (blue) was used to stain the nucleus (n = 5). ( B ) BCP mice were treated with intraperitoneal (i.p.) injection of DMXAA twice on days 3 and 6. The Paw withdrawal threshold (PWT) of mice was detected on 0, 3 (before DMXAA injection), 6 (before DMXAA injection), 10, and 14 days after the surgury in sham, BCP, BCP + vehicle, and BCP + DMXAA groups. DMXAA treatment attenuated hypersensitivity induced by BCP ( P < 0.05 vs. the sham; P < 0.05 vs. the BCP + vehicle; n = 10; mean ± SEM; Two-way Repeated Measures ANOVA followed by Tukey’s post-hoc test). ( C ) Quantitative analysis of STING expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + vehicle; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of the sham, BCP, BCP + vehicle, and BCP + DMXAA groups on day 7 post-operation. α-Tubulin and GAPDH were used as loading controls, respectively. n represents the number of experimental animals in each group.

    Article Snippet: The antibodies used were as follow: mouse anti-AIM2 monoclonal antibody (1:300; cat. no.: 66902-1-IG, RRID: AB_2882230; Proteintech, USA) ; rabbit anti-AIM2 polyclonal antibody (1:300; cat. no.: bs-5986R, RRID: AB_11074508; Bioss, China) ; mouse anti-GluN1 monoclonal antibody (1:300; cat. no.: 32-0500, RRID: AB_86917; Thermo Fisher Scientific, USA) ; rabbit anti-STING polyclonal antibody (1:300; cat. no.: 19851-1-AP, RRID: AB_10665370; Proteintech, USA) ; mouse anti-Synapsin1 monoclonal antibody (1:300; cat. no.: MA5-31919, RRID: AB_2787542; Thermo Fisher Scientific, USA); guinea pig anti-MAP2 monoclonal antibody (1:300; cat. no.: 188004, RRID: AB_2138181; Synaptic Systems, Germany); mouse anti-Iba1 antibody (1:200; cat. no.: sc-32725, RRID: AB_667733; Santa Cruz, USA).

    Techniques: Staining, Injection, Expressing, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR

    Decrease of absent in melanoma 2 (AIM2) promotes the expression of stimulator of interferon gene (STING) in the spinal dorsal horn. ( A ) In the spinal dorsal horn of the wild type (WT) mice, AIM2 (green), STING (red), and microtubule-associated protein 2 (MAP2) (rose red) were used for triple immunofluorescent. DAPI (blue) was used to stain the nucleus (n = 5). Merged images show the location of AIM2 and STING in dorsal horn neurons. ( B ) Immunofluorescence staining with anti-STING (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-Lentivirus (LV), and BCP + AIM2-LV groups. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( C ) Quantitative analysis of STING expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups on day 7 post-operation. α-Tubulin and GAPDH were used as loading controls, respectively. n represents the number of experimental animals in each group.

    Journal: Scientific Reports

    Article Title: AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice

    doi: 10.1038/s41598-024-83027-w

    Figure Lengend Snippet: Decrease of absent in melanoma 2 (AIM2) promotes the expression of stimulator of interferon gene (STING) in the spinal dorsal horn. ( A ) In the spinal dorsal horn of the wild type (WT) mice, AIM2 (green), STING (red), and microtubule-associated protein 2 (MAP2) (rose red) were used for triple immunofluorescent. DAPI (blue) was used to stain the nucleus (n = 5). Merged images show the location of AIM2 and STING in dorsal horn neurons. ( B ) Immunofluorescence staining with anti-STING (red) in the spinal dorsal horn of the sham, BCP, BCP + CON-Lentivirus (LV), and BCP + AIM2-LV groups. DAPI (blue) was used to stain the nucleus. The precise injection site of the virus in the dorsal horn was indicated by the green fluorescence, which represented the neuron transfected with lentivirus (n = 5). ( C ) Quantitative analysis of STING expression by western blot (WB) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) (* P < 0.05 vs. the sham; # P < 0.05 vs. the BCP + CON-LV; n = 5; mean ± SEM; one-way ANOVA followed by Tukey’s post-hoc test) in the spinal cords of sham, BCP, BCP + CON-LV, and BCP + AIM2-LV groups on day 7 post-operation. α-Tubulin and GAPDH were used as loading controls, respectively. n represents the number of experimental animals in each group.

    Article Snippet: The antibodies used were as follow: mouse anti-AIM2 monoclonal antibody (1:300; cat. no.: 66902-1-IG, RRID: AB_2882230; Proteintech, USA) ; rabbit anti-AIM2 polyclonal antibody (1:300; cat. no.: bs-5986R, RRID: AB_11074508; Bioss, China) ; mouse anti-GluN1 monoclonal antibody (1:300; cat. no.: 32-0500, RRID: AB_86917; Thermo Fisher Scientific, USA) ; rabbit anti-STING polyclonal antibody (1:300; cat. no.: 19851-1-AP, RRID: AB_10665370; Proteintech, USA) ; mouse anti-Synapsin1 monoclonal antibody (1:300; cat. no.: MA5-31919, RRID: AB_2787542; Thermo Fisher Scientific, USA); guinea pig anti-MAP2 monoclonal antibody (1:300; cat. no.: 188004, RRID: AB_2138181; Synaptic Systems, Germany); mouse anti-Iba1 antibody (1:200; cat. no.: sc-32725, RRID: AB_667733; Santa Cruz, USA).

    Techniques: Expressing, Staining, Immunofluorescence, Injection, Virus, Fluorescence, Transfection, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR